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Infusions (0.1 min5 min) were done during the first spontaneous dark-phase meal.
Previous studies have shown two limitations: (i) cell cultures of HPMC lasted for days only when incubated in culture medium and (ii) short-term studies of <30 min were done in HPMC when incubated with peritoneal dialysis fluid (PDF).
Washes (4×10 min) were done in TBST containing 1% Triton X-100 and increasing concentrations of NaCl (250, 500, 750 mM and 1M).
Clinical and spirometric parameters were monitored as early as 1-min post administration of the drug and frequent observations at 5, 15, 30 and 60 min were done.
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A final elongation step at 72 °C for 5 min was done to complete the reaction.
Subsequent to the growth, the catalyst was removed, and in some cases, a rapid thermal annealing at 750°C for 5 min was done to activate dopant impurities.
A debrief period of 5 to 10 min was done after each practice session in order to give positive or corrective feedback as indicated.
Ultrasonic cleaning in acetone, methanol and deionized (DI) water, respectively, for about 15 min was done after polishing to clean the surface more effectively and then dried with (N2) nitrogen stream.
The assessment of the FDG-PET/CT scans performed at 60 min was done twice in a blinded fashion with 2 months in between by author MHV in order to investigate the intra-observer repeatability at post imaging processing.
Following washes with PBS, permeabilization using 6 N HCl for 7 min was done, which were then neutralised with 0.1 M Borate buffer (pH 8.5) [31].
The novel object test, which lasted 5 min, was done after the open field test.
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