Your English writing platform
Discover LudwigSuggestions(5)
Exact(11)
Therapy sessions each lasting 60 90 min were carried out on 10 successive days.
Seventy cycles of stepwise program (94°C for 1 min, 50°C for 2 min and 72°C for 3 min) were carried out.
After an initial 15 min denaturation at 94°C, 35 cycles of 94°C for 1 min, 65°C for 30 sec and 72°C for 1 min were carried out, followed by a 5 min extension at 72°C using a thermal cycler (Tec-571, Techne, UK).
One initial denaturation at 94°C, 2 min and one final extension at 72°C, 5 min were carried out in all amplifications.
Then, 30 cycles of denaturation (at 95°C for 30 sec), annealing (at 50°C for 50 seconds), and extension (at 72°C for 1 min) were carried out.
The assay was performed as previously described (Shannon et al, 2008), except that incubation with primary and secondary antibodies (2 h each, with agitation) and the colour development step (30 min) were carried out at 37°C.
Similar(49)
After this, a final extension at 72 °C for 10 min was carried out.
For some of the thin films, thermal annealing at 155 °C for 30 min was carried out in a vacuum.
Clear pumping for 10 min was carried out until stable meter readings of the in-situ parameters (temperature, pH, Eh and EC) was obtained using portable digital meters.
For this aim, a rapid thermal oxidation (RTO) at 900°C for 10 min was carried out in a Jipelec JetFirst furnace.
Secondly, a soft bake process at 40°C for 30 min and 120°C for 2 min was carried out, followed by exposure performed by a Karl Suss MJB3 contact aligner (SUSS MicroTec, Garching, Germany) for 80 s at 150 mW/cm2.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com