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After 15 min of oxygen plasma treatment, the substrates were preheated at 75 °C for 10 min on a heating plate.

In both methodological approaches we observed rapid TJ protein disruptions after 30 min of oxygen and glucose deprivation or middle cerebral artery occlusion, which were accompanied by strong caspase-3 activation in brain endothelial cells (BEC).

Significantly (P < 0.01), fewer Δ yfh1 cells were stained after 60 min than 15 min of oxygen exposition.

Although the expression of these genes in anaerobiosis and aerobiosis was not altered in Δ yfh1 compared with wild-type cells, they were repressed after 30 min of oxygen exposition (Table  1).

HBO treatment was provided using pressure/time schedules selected from existing protocols by each participating centre, aimed at delivering a dose approximately bioequivalent to 90 min of oxygen breathing at 2.4 atmospheres absolute pressure.

After 30 min of oxygen exposition, a significant (P < 0.01) increase in the number of cells in G1 and a decrease in the number of cells in G2/M were visible (Fig.  6B).

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A premixed flame fueled by 1.19 L min-1 of methane and 2.46 L min-1 of oxygen was ignited and maintained to support the combustion of the spray.

The precursor solution was injected at 5 mL min-1 through the reactor nozzle and dispersed with 5.0 L min-1 of oxygen into a fine spray (5/5 flame) while maintaining a constant pressure drop of 1.5 bar across the nozzle tip.

The plate and measuring cartridge (containing waveguides and fluorescent oxygen sensors together with reagents for automated pneumatic additions of inhibitors and uncouplers) was loaded into the machine and after the calibration procedure (30 min calibration, 10 min rest), measurement cycles of 1 min sample mixing, 2 min waiting and 3 min measurement of oxygen consumption rate were initiated.

Mean SaO2 value after 5 min administration of oxygen-enriched air was not significantly different from that of medical air.

In the operating room, we started continuous electrocardiogram (ECG) on standard lead II, automated noninvasive measurement of blood pressure every 5 min, and measurement of oxygen saturation by pulse oximetry (SpO2).

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