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Placental tissues were obtained within 15 min of delivery from all of the subjects.

In a group of pregnancies the placenta was collected within 30 min of delivery.

However, recombinant ASM delivered in vivo (in mice) is rapidly cleared from circulation, 40% within 1 min of delivery.

Within 30 min of delivery, maternal and fetal circulations were established independently to a peripheral lobule (21).

The samples were immediately (within 5 min of delivery) frozen in liquid nitrogen to minimize hypoxic/ischemic artifacts (20).

1,960 (95%%) neonates breathed within 1 min of delivery; 44 (2 %) and 3 (0.1 %) of them required 1 2 min and >2 min, respectively, to begin breathing.

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Similar(48)

We conclude that the optimal window for collecting placental tissue without incurring metabolic artifacts due to hypoxic conditions is within 9 min of placental delivery.

After written informed consent, placenta was collected and processed within 15 min of the delivery.

For the placenta, small pieces (<150 mg) were dissected from the villus tree within 15 min of the delivery.

An IUD (Pregna Copper T 380A; Pregna International, Chakan, India) was placed in the fundus of the uterus by one of the authors within 10 min of placental delivery.

As can be seen, during the first ∼60 min of insulin delivery, the TPR declined to a level of 83.8 ± 3.6 and 78.5 ± 5.0% for MD and MP catheters, respectively, and then remained at these levels until the end of the 6-h period of variable insulin delivery.

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