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All protective solutions, including skim milk and sugars were sterilized at 121 °C for 15 min before experiments.
Cells were returned to STS-free solutions 5 min before experiments.
To activate the photosynthetic activity, detached leaves were exposed to an illumination of 1000 μmol m-2 s-1 for 30 min before experiments.
Cells were incubated with 5 μM MgGreen in presence of 0.02 % pluronic F127 for 20 min and gently washed 15 min before experiments.
The PDMS-based microfluidic devices were placed in vacuum for at least 30 min before experiments were run to reduce priming issues.
For some experiments, Ca2+ was depleted from intracellular stores by pre-incubating the cells for 5 min before experiments commenced in the NMDG buffer supplemented with 1 µM thapsigargin.
Similar(52)
The positive control group received diazepam (1 mg/kg) was also administered the intraperitoneal (0.1 mL/mouse) 30 min before the experiments.
Acclimatization of over 5 min before the experiments ensured that the temperature-sensitive endocytotic shibireTS1-function should either block (30°C, restrictive temperature) or allow (19°C, permissive temperature) signaling [3] via L2-R feedback connections.
The coating was performed at least 45 min before the experiments, and the Pluronic was completely removed.
Mice were placed into a transparent observation chamber (30 × 30 × 25 cm) for adaptation 30 min before the experiments (Kim et al., 2001).
Once electrodes were implanted into the brain, the potential (−650 mV) was applied and the CPEs were left to settle for 30 min before any experiments began.
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