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Approximately 30 min before trials were initiated, adult H. parallelus were placed into individual holding/release tubes and isolated from possible semiochemical sources.
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Now there was a delay of 1, 15 or 90 min before Trial 7, when a novel object was paired with the highly familiar object ('novel object vs. highly familiar object recognition').
Females were also taken from their housing cages 10 min before the trials in which they participated, and at the start of the trials were immediately released into the mate choice aviary and video recorded for one hour via four cameras, each focused on one male cage.
To determine the effects of inhibitors on AS-induced hypertrophy, the myotubes were treated with or without inhibitors (wortmannin or rapamycin) 30 min before the trials.
MEXX was administered 60 min before the trial, and scopolamine was injected 30 min before the trial.
Each mouse was administered imipramine hydrochloride (20 mg/kg, dissolved in saline) or vehicle i.p. 30 min before the trial.
As shown in Figure 4, Ca2+ release by uncaging of NP-EGTA-AM 5 min before one-trial conditioning also induced a specific LTM increase at 72 h.
The samples were let to stand at room temperature for 30 min before starting the trials.
20 min before each daily trial under ad lib or food-deprived conditions.
The greyhounds were pre-treated orally with either an empty gel capsule (sham) or 1 mg/kg diazepam 90 min before the third trial (n = 39).
Mice were acclimatized to the apparatus for 5 min before the first trial began.
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