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When we tested a single dose (30 min before tests) of either antagonist in mice treated chronically with ethanol, both antagonists attenuated ethanol-induced effects.
After establishment of the whole-cell patch configuration, the cells were allowed to stabilize for 3 5 min before tests.
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Animals were allowed to habituate to the apparatus (Plantar Test Apparatus, Stoelting, IL, US) for 10 15 min before testing began.
The solution was equilibrated for about 5 min before test.
All animals were acclimatized to the test chamber 30 min before testing.
It should be pointed out that all samples are sonicated for 20 min before testing.
They were transferred to a bioassay room, and allowed to acclimate for at least 30 min, before testing.
Escalating doses of cortisol (0, 3, 6, 12, 24 mg) were administered 8 min before testing according to a between-subjects design.
It had no effect when administered 30 min before each conditioning session during CPP training period (acquisition) or 30 min before testing for place preference in the absence of morphine (expression).
Participants were divided into two groups; (i) experimental group consumed 250 ml of Black Tea (BT) 40 min before testing, assuming caffeine at a dose of 50 mg plus L-theanine at a dose of 15 mg while (ii) control group consumed 250 ml of water (i.e., drinking water; not boiled or distilled).
Mice were brought to the laboratory 60 min before testing between 9.00 AM and 6.00 PM.
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