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Stimulations were scheduled 80 min before lights off.
MTII (80 µg; H-3902, Bachem, Torrence, CA) or 100 µl saline was injected intraperitoneally, 30 min before lights out and food intake was monitored for 24 h [14].
Drug injections then resumed for 33 out of the next 35 days and ATN-224 was given 15 min before lights went off at night.
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Apyrase was applied for 30 min before light-stimulation of astrocytes.
The baseline period was defined as 30 min before light onset, excluding the minute before light onset.
When the bioreductive drug was included in the treatment, administered 20 min before light irradiation, regrowth delay was greatly increased.
Groups of rats with chondrosarcoma were given 2 mg kg−1 of BPD intravenously 5 min to 180 min before light treatment of 150 J cm−2 690 nm.
Anaesthetised mice were injected intraperitoneally with 3 mg of D-Luciferin (Promega, Fitchburg, WI, USA) diluted in PBS, 5 min before light caption.
When interstitial PDT was combined with a low dose of MMC (administered 15 min before illumination), the Photofrin dose or light dose could be reduced by a factor of 2 in order to obtain equivalent cure rate or growth delay.
Finally, the chemiluminescence substrate CDP-Star (Tropix, Bedford, USA) was applied and incubated on the membrane for 5 min before the light signals were detected in a camera using the analysis software Quantity One (Bio-Rad, Munich, Germany).
The administration of oxypurinol at 30 min before PDT light treatment completely prevented the PDT-induced burst in XO activity, while oxypurinol has not significantly affected the activity of this enzyme in nontreated tumours.
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