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Drugs were dissolved in the recording EBS and cultures were pre-incubated in this solution for 10 minutes before experiments were performed.
HMVECs were preincubated with different concentrations of certolizumab pegol or its Ms-Ig control for 30 minutes before experiments.
The slices were allowed to recover for 30 min, before being transferred to ACSF without choline chloride for an additional 30+ minutes before experiments commenced.
Ninety minutes before experiments, MO3.13 cells were deprived of serum and after 1 h stained for 1 h with TMRM 50 nM and mounted into the incubator chamber for the acquisition in time-lapse at 37 °C in a humidified atmosphere containing 5% CO2.
Similar(56)
Drugs were applied 15 minutes before experiment.
For all experiments, cells were first treated with Mitotracker Deep Red (Invitrogen) for 45 minutes before passaging with trypsin.
I'd seen him five minutes before".
Stretch for ten minutes before jogging.
Eyes were kept in BSS solution at room temperature with drug for 20 minutes before beginning experiments.
When the temperature control was switched on, the whole system as well as the sample was left for equilibrium for at least 30 minutes before the experiments were conducted.
The mouse was anesthetized 5 minutes before the experiments by intraperitoneal (IP) injection of a cocktail containing Ketamine (54 mg/kg body weight) and Xylazine (6 mg/kg body weight).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com