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The laser, pinhole and gain settings of the confocal microscope were kept identical among different treatments.
For semi-quantitative measurement of fluorescence intensities, laser, pinhole and gain settings of the confocal microscope were kept identical among treatments.
Since fluorescence intensity was quantified for each image, the gain and offset settings of the microscope were kept constant over the duration of each experiment.
The imaging box and microscope were kept at 36.5°C by a climate chamber that surrounds the whole stage of the microscope, including the objectives.
All optical settings of the microscope were kept constant for all image acquisitions, in order to be able to compare the acquired information.
Similar(55)
The pinhole of the confocal microscope was kept at 50 μm.
In order to obtain maximum stability of the mechanical set-up, the microscope was kept working overnight before the relevant measurements were performed.
The distance from the cornea to the microscope was kept stable using a single-use contact element in sterile packaging, (TomoCap, Heidelberg Engineering, Heidelberg, Germany).
Spleen, pancreas and liver precursors for transplantation were extracted under a light microscope and were kept in sterile conditions at 4°C in RPMI 1640 (Biological Industries, Beit HaEmek, Israel) prior to transplantation.
All the microscope settings were kept consistent in each experiment.
When comparing aggregate load between genotypes, all microscope settings were kept constant.
Related(20)
light were kept
illumination were kept
use were kept
magnification were kept
microscope were analyzed
microscope were reconstructed
microscope were analysed
microscope were carried
microscope were found
microscope were chosen
microscope were processed
microscope were collected
microscope were performed
microscope were photographed
microscope were captured
microscope were judged
microscope were defined
microscope were purged
microscope were made
microscope were relocated
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