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Raw data obtained from the microscope were processed in Gwyddion open source software using the plane leveling algorithm [19].
The images acquired from the confocal microscope were processed using Corel Photo-Paint software (Corel Corporation, Dallas, TX, USA).
Coverslips were mounted in UltraCruz mounting medium (Santa Cruz, Heidelberg, Germany) and images obtained with a Leica florescence microscope were processed using MetaMorph software (Molecular Devices, Biberach, Germany).
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With this method, detectors are evaluated by determining circumferential tracks, and digital images of scanning electron and optical microscopes are processed by using the Hough Transform; the resulting circumference parameters are refined using the Max Min clustering algorithm.
Images were acquired using a Zeiss fluorescence microscope or a Leica SP5 confocal microscope and were processed using Photoshop (Adobe) software.
Images were acquired on a Zeiss LSM710 confocal microscope and were processed using Adobe Photoshop.
Confocal microscope images were processed with Matlab (TU Delft, The Netherlands) and Image J (National Institutes of Health).
All light photography was done using a Spot II camera attached to a Leica dissecting microscope; images were processed with Adobe Photoshop.
Images of living cells were acquired with a CoolSnap EZ (Roper) camera using a 63 × /1.4 Plan Apochromat oil objective on a Zeiss AxioImager microscope and were processed with MetaMorph software (Molecular Devices Corporation).
Slides were observed and photographed by Evolution MP5.0 colour Media Cybernetics camera in conjunction with a Leica DMRB microscope and images were processed using Photoshop CS6.
Fluorescent signals were detected using a Zeiss inverted microscope and images were processed with Photoshop CS3 (Adobe Systems).
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