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The distinctive representative characteristics under normal light and polarized light microscope were chosen and imaged.
Images which were not affected by fluorescence quenching due to the light-exposure in microscope were chosen for computer analysis.
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A confocal microscope is chosen to analyse the 3D deformation profiles of the device at different temperature-induced stress states.
Zoom factor of the microscope was chosen equal or greater to the required Nyquist criterion to ensure the focal spot overlaps between neighboring lines.
This microscope was chosen because it enabled observation of individual colonies growing directly on streaked plates without requiring the preparation of specialized agar-coated microscope slides or any physical disruption of the colonies or agar.
For the hydrated biofilms of interest in these studies (examined in situ), quartz microscope slides were chosen in order to avoid the high levels of fluorescence associated with using NIR illumination on a glass substrate.
If Ki67 expression was uniformly distributed over the entire slide, 500 2000 cells were chosen from different microscope views; otherwise, 2000 cells were equally counted in both hotspot and negative areas in slice.
From each cestode, 2 4 gravid proglottids were chosen and analyzed using a microscope.
Representative areas were chosen for ultrathin sectioning and examined with an electron microscope (JEM 1010 transmission electron microscope; JEOL, Peabody, MA, USA) as described previously.
Representative areas were chosen for ultrathin sectioning and examined on a transmission electron microscope at a magnification of × 10 000.
Representative areas were chosen for ultra-thin sections to view with a JEM-1400 electron microscope (JEOL Ltd., Tokyo, Japan).
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