Microscope slides were analysed using a Leica TCS NT confocal microscope (Leica Microsystems) using a 63× immersion objective lens.
The prepared microscope slides were analysed in 12 transects, each 4 mm apart, to identify pollen and tephra grains and to ascertain the approximate time of the capture of various particles by the trap.
The sections were examined for neuropathology using a Nikon Eclipse 80i light microscope and were analysed by a blinded investigator.
In both cases, samples were visualized using a PerkinElmer Improvision Ultraview VoX Spinning disc confocal microscope and were analysed with Improvision Volocity 5.3.1 and ImageJ 1.42q.
Nematodes were visualized on a Carl Zeiss Axiovert 200m inverted microscope and images were analysed by Metamorph software (version 6.3r7).
Image acquisition was performed with an Olympus BX50 epi-fluorescence microscope and images were analysed with the Image-Pro® Plus Softhere Olympus Stream Image Analysis SoftwareOlympus Stream Image Analysis Software
For quantification, the wells were imaged using a microscope, and the colonies were analysed using ImageJ software.
Samples of rat brain tissues were analysed by microscope, and it was found that all silica-based nanoparticles permeated to the brain tissue.
Following normal dehydration, lucidification and mounting the slides were analysed under microscope (Olympus, Tokyo, Japan) as specified in our previous studies [ 22- 25] and digital images were captured with camera (Olympus, Tokyo, Japan).
Preparations were analysed by microscope and when technically possible, all CK+ cells detected by their cytoplasmic fluorescence were photographed under FITC, and, in the case of Cyclin D1 or myc hybridisation, under SpectrumOrange excitations.
