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Exact(5)
Overall, approximately 5% of the transcripts found to be present in the microarray were absent according to RT-PCR, and 30 40% of absent determinations according to the microarray were detected by RT-PCR (Table S1).
No transcriptional changes by microarray were detected for Hspb1 (Hsp25), but RT-PCR showed increases after a 7 day WY exposure in a previous study [ 12].
Overall, 27,857 out of the 39,015 transcripts on the microarray were detected at least in one of the eleven stages (Additional file 2).
Meanwhile, no non-specific hybridization signals between the HBV DNA and the chip probes (NimbleGen HG18 RefSeq Promoter Microarray) were detected.
To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay.
Similar(55)
Fluorescent DNA bound to the microarray was detected with a GenePix 4000B scanner (Axon Instruments, Foster city, CA), using the GenePix 4000 software package to locate signal from spots.
IL-11, which demonstrated as differential expression in microarray, was detected by real-time PCR, western blot, ELISA and immunohistochemistry staining.
The signal intensity for each probe set on the GeneChip microarray was detected with a GeneChip® Scanner 3000 (Affymetrix, Cleveland, USA), and the raw signal value for each probe set was analyzed with GeneChip operating software (GCOS; Affymetrix, Cleveland, USA).
The amount of phage particles at each spot throughout the microarray was detected using a mouse monoclonal antibody to the T7 capsid protein and quantified using Alexa Fluor 532 (green-fluorescent dye -labeled goat anti-mouse antibodye -labeled
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent's Microarray Scanner System.
Signals on the microarrays were detected with the Agilent DNA Microarray Scanner.
Related(20)
microarray were ascertained
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microarray were identified
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microarray were employed
microarray were isolated
microarray were investigated
microarray were obtained
microarray were normalized
microarray were quantified
microarray were taken
microarray was detected
microarray were done
microarray were found
microarray were validated
microarray were considered
microarray were plotted
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