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Overall, approximately 5% of the transcripts found to be present in the microarray were absent according to RT-PCR, and 30 40% of absent determinations according to the microarray were detected by RT-PCR (Table S1).

No transcriptional changes by microarray were detected for Hspb1 (Hsp25), but RT-PCR showed increases after a 7 day WY exposure in a previous study [ 12].

Overall, 27,857 out of the 39,015 transcripts on the microarray were detected at least in one of the eleven stages (Additional file 2).

Meanwhile, no non-specific hybridization signals between the HBV DNA and the chip probes (NimbleGen HG18 RefSeq Promoter Microarray) were detected.

To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay.

Similar(55)

Fluorescent DNA bound to the microarray was detected with a GenePix 4000B scanner (Axon Instruments, Foster city, CA), using the GenePix 4000 software package to locate signal from spots.

IL-11, which demonstrated as differential expression in microarray, was detected by real-time PCR, western blot, ELISA and immunohistochemistry staining.

The signal intensity for each probe set on the GeneChip microarray was detected with a GeneChip® Scanner 3000 (Affymetrix, Cleveland, USA), and the raw signal value for each probe set was analyzed with GeneChip operating software (GCOS; Affymetrix, Cleveland, USA).

The amount of phage particles at each spot throughout the microarray was detected using a mouse monoclonal antibody to the T7 capsid protein and quantified using Alexa Fluor 532 (green-fluorescent dye -labeled goat anti-mouse antibodye -labeled

Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent's Microarray Scanner System.

Signals on the microarrays were detected with the Agilent DNA Microarray Scanner.

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