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I calculated average observed and expected heterozygosity using all 61,435 SNPs from the microarray because the vast majority of the loci in the microarray were ascertained by dog-dog comparisons, and again using only the 16 high-heterozygosity SNPs genotyped in this study.
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The reproducibility of the microarray was ascertained by comparing samples from bovine PBLs with ConA activated PBLs using replicates and dye swaps.
Afterwards, the results were ascertained with an ANOVA based microarray analysis, GeneANOVA, which is a freely available gene expression analysis of variance software developed by The Laboratory of "Génome et Informatique" at Evry-Génopole [ 109].
Treatment and follow-up information were ascertained.
Demographic and clinical characteristics were ascertained.
Human SNPs were ascertained in three ways.
Treatment outcomes were ascertained for 225 persons.
Pedigrees were ascertained through a GD proband.
Multiple covariates were ascertained.
515 cardiovascular events were ascertained.
Improvements from 2.667 to 31.60% were ascertained.
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