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Additionally, a combination treatment of JH + SHE that was not included in microarray studies was evaluated.
ICAM-1 expression from independent published microarray studies was extracted from Oncomine database (http://www.oncomine.org).org
A further difference highlighted in this study between bacterial and eukaryotic CGH microarray studies was the effect of spatial correlation.
The remainder of the hippocampal total RNA extracts not used in the microarray studies was used for real-time PCR.
The significant upregulation of p21/CDKN1A CDKs inhibitor gene, monitored by microarray studies, was further investigated at the protein level by immunoblot analysis.
However, the increase in the expression of LOX and other matrix components observed in these microarray studies was not subsequently validated using other techniques (Henegar et al., 2008).
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The utility and a method for integrating data within and across DNA microarray studies is shown in an example analysis using DrugMatrix data deposited in CEBS by Iconix Pharmaceuticals.
If the genes identified as sperm quality markers by microarray studies are successfully attributed to the pathogenesis of male infertility, then the microarray strategy may be used as a clinical diagnostic tool for male infertility.
Detail results of the microarray studies are unpublished.
Microarray studies were done in three biological independent triplicates.
Mice used for microarray studies were not subjected to behavioral testing.
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