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For analyzing Sulf expression patterns in published gene microarray studies, we used the oncomine database (www.oncomine.org) and http://source.Stanford.edu.edu
To confirm and/or extend some of the major findings in the microarray studies, we have analyzed three specific components: MUC5AC expression, IL-8 secretion, and cholesterol metabolism.
In our experience of post-array confirmation of similar sets of targets derived from sscDNA-based microarray studies, we have been able to confirm by qPCR the vast majority of these.
As a prelude to microarray studies, we used a candidate gene approach, along with real-time polymerase chain reaction (qPCR), to evaluate potential differences in gene expression between summer and migratory butterflies using whole head homogenates.
Compared with the previous microarray studies, we identified 48 down-regulated probes in both the Otx2 CKO and Nrl KO retinas, while at the same time, 18 probes were down-regulated in the Otx2 CKO but up-regulated in the Nrl KO retina.
To overcome the limitations associated with microarray studies, we used "next generation" sequencing technology (specifically massively parallel pyrosequencing using the 454 Life Sciences platform) of small RNA cDNA libraries to more comprehensively characterize miRNA expression in epithelial ovarian cancer, as well as in primary cultures of normal ovarian surface epithelial cells.
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In a previous microarray study, we compared primary lung adenocarcinoma (AC) with squamous cell carcinoma (SCC) in order to find new immunohistochemical antibodies that could improve the accuracy of the distinction in daily practice [3].
To check the accuracy of miR-425* expression pattern obtained from microarray study, we used Taqman based miRNA RT-qPCR assays as a golden-standard platform to quantify the levels of plasma miRNAs in the same 20 samples.
Based on the pathway analysis findings from the microarray study, we hypothesized that PT would suppress recruitment of immune cells to the airways by reducing the expression of proinflammatory cytokines and chemokines.
In the present microarray study, we also found downregulation of brain-derived neurotrophic factor (BDNF).
In a previous microarray study, we demonstrated that miR-130b was upregulated in esophageal squamous cell carcinoma (ESCC) tissues.
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