Microarray studies were done in three biological independent triplicates.
Mice used for microarray studies were not subjected to behavioral testing.
To address this question, microarray studies were performed to analyze the gene expression profile of this mutant line.
All these previous microarray studies were originally designed for the identification of differentially expressed genes between normal and malignant tumor tissues for that specific type of cancer.
Microarray studies were previously used to identify multiple novel hematopoietic and vascular genes misexpressed in the cloche mutant [12], [13], [14].
Potential gene targets identified through subtractive hybridization [29] and microarray studies, were searched for classic nuclear receptor response element binding sites.
Gene expression from 15 of the 90 axon growth promoting proteins identified in the microarray studies were analyzed using qPCR methods.
The resulting phenotypes were characterized and microarray studies were conducted on 24 hpf zebrafish embryos microinjected with the most potent of the two MOs, prp2-MO2.
Although our microarray studies were extensive (28 arrays for 4 conditions), we ran qPCR to validate the microarray findings, focusing on virulence genes that are biomarkers of the bacterial switch to a hypervirulent phenotype.
Clones that were differentially regulated in Ms and Hv larvae in response to feeding on defenseless plants, as identified by microarray studies, were selected and subjected to single-pass sequencing using a 5' vector primer.
