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We prospectively validated the microarray findings in independent samples.
Real-time PCR was used to validate the microarray findings in the SK-N-Be(2 C cell lines.
One report has described microarray findings in MS brain lesions using autopsy samples from human subjects [ 8].
Consistent with our interpretation of the microarray findings in KIKO skeletal muscle, we found a coordinate downregulation of Pgc1a and upregulation of Srebp1 (Fig. 2A,C,D).
The aim of the study was to perform preliminary biological validation of gene expression microarray findings in human disease, and to search for putative feasible biomarkers for disease diagnostics.
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Along with the microarray findings presented in Tables 1 and 2, and compared to appropriate controls, these data suggest GIPC1 suppression promotes cell division arrest and apoptosis.
However, our microarray findings implicated Hunk in intestinal tumourigenesis, a role we wished to elucidate further.
We next evaluated the cell cycle phases in U87 and A172 cells in order to corroborate the microarray findings of differentially expressed genes in pathways related to cell cycle regulation.
We were able to confirm several of the original microarray findings, even surprising ones, in human cervical disease, which is the result of concerted action of viral proteins in the epithelium.
Mirroring the microarray findings, the hormonal message in upper SI K-cells was dominated by gip, but the cells additionally contained detectable mRNA for sst, sct, cck, gcg, and pyy.
Microarray findings suggest GIPC1 is involved in adhesion and motility (Tables 1 and 2).
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