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Microarray analyses in yeast and human cells have demonstrated dramatic effects of rapamycin on gene expression [6] [10].
In microarray analyses in Senecio allopolyploids, quantitative RT-PCR confirmation rates were also approximately 65% [25], [26].
In the present work we employed microarray analyses in order to identify the common gene expression profile in bladder cancer.
To confirm that the PKA and TOR pathways were indeed inhibiting Ste11 activity, we performed microarray analyses in cells growing in rich medium after Tor2 inactivation.
We have previously subjected CREB-silenced A375SM cells to cDNA microarray analyses in order to identify downstream target genes regulated by CREB in melanoma [7].
Here, therefore, we examined inflammatory cytokines in the serum, and inflammatory signaling in the brain using hippocampal microarray analyses in the context of normal aging, and at low doses of PIO.
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This should circumvent the limits of the microarray analysed in this study, particularly the reduced number of genes that were screened.
Microarray analyses used in-house (Breakthrough Breast Cancer Research Centre, London, UK) human arrays spotted with DNA derived from 4,600 IMAGE cDNA clones in duplicate.
The characteristics of the six patients analysed by microarray are described in table 1. Histology of lung tissues analysed in the microarray analyses were shown in figure S2 in the online data supplement.
KS designed and carried out the experimental infections, carried out the microarray analyses, participated in the biological interpretation and in drafting the manuscript.
Our microarray analyses are in line with previous proteomic experiments performed in primary gingival epithelial cells (GEC) [ 44].
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