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Finally, similar analyses could be performed on other microarray platforms in the future to allow a cross-platform comparison.
However, the microRNA annotation version used for the microarray probe design was different among the five different microarray platforms in April 2008.
Advances in microarray technology have enabled exon-specific microarray platforms, in which all known and predicted exons in the genome have hybridization probes.
Microarray platforms, in particular, have given enormous momentum to the study of brain gene regulation in the contexts of sensory/motor processing, learning, the formation of memories, aging, and the onset of diseases [1] [6].
Thus, both unsupervised hierarchical clustering and modular analyses confirmed the robustness of the gene expression profile in PBMC of S. aureus-infected patients across both the Affymetrix and Illumina BeadChip microarray platforms in distinct groups of subjects.
Notably, several recent studies using the International HapMap Project (http://www.hapmap.org/) [2], [3] lymphoblastoid cell lines (LCLs) demonstrated the utility of these microarray platforms in profiling gene expression and dissecting the genetic architecture of gene regulation [4].
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Our study is consistent with, but provides complementary insight to previous seminal work that demonstrated the reproducibility of various gene expression profiles, when multiple array cohorts were run simultaneously, using the same protocol, on the same microarray platform in different laboratories [30].
Analysis of differentially expressed miRNAs according the microarray platform in IDL and DP-induced leaves.
Here, we used a microarray platform in Drosophila serrata to measure 11,604 gene expression phenotypes.
A similar approach has already provided robust and flexible microarray platform in other fish species [ 10- 18].
The use of a different microarray platform in the measurement of the transcriptional profile could also be a contributing factor.
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