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When the researchers injected normal mice with bicarbonate--a buffer that prevents the pH dip--in the amygdala, the mice froze less in response to CO2.

Post-hoc analysis of contextual freezing demonstrated that vehicle-treated mice froze significantly less than sham-treated controls, and muscimol-treated mice froze less to context exposure than vehicle-treated mice or sham controls (Figure 2A).

Analysis of freezing in response to the tone presentation yielded a significant main effect of Group, [F 1,56) = 14.21, p<0.0005], and a significant Group by Minutes interaction, [F 7,392) = 2.19, p = 0.048] showing that the PCP-treated mice froze less than the controls but the magnitude of the differences was time dependent.

Overall, CRND8 mice froze less during the whole test than nTg mice (F 1,73) = 36.2, P < 0.001, genotype effect).

The six- and 12-month-old CRND8 mice froze less than their three-month-old counterparts (P = 0.1 and P = 0.07, respectively, Bonferroni t-test).

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Specifically, the PCP-treated mice froze significantly less often than the control group for minutes 1, 2, and 8 (p<0.006), while very large differences were also observed for minutes 6, 7, and 9 (p<0.025) (Fig. 5C).

Post-hoc analysis revealed that in both contextual and CS freezing muscimol infused mice froze significantly less than vehicle controls, and that vehicle infusion significantly impaired contextual learning compared to sham controls, while a trend toward a deficit was observed in CS freezing.

The PCP-treated mice froze significantly less often than the controls in response to the contextual cues for each of the first 4 min of the test session (p<0.0006) while differences were also substantial for min 5 (p = 0.040) and 6 (p = 0.025).

Both genotypes exhibited high levels of cue-evoked freezing during CS+ trials and low levels of freezing during CS− trials, but 5-HTTOE mice froze significantly less than WTs during CS20% trials.

The post-hoc analysis revealed that CRND8 mice froze significantly less during the exploration of the altered training chamber than nTg mice (F 1,73) = 12.6, P < 0.001, genotype effect, Figure 1C left panel).

Post-hoc comparisons revealed that 12-month-old CRND8 mice froze significantly less than their nTg littermates (t(22) = 3.4, P < 0.01); however, the contextual memory of three- and six-month-old CRND8 and nTg mice was comparable.

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