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Direct imaging of the tumour metabolic activity using 18F-FDG PET/CT is widely applicable but has a relatively low sensitivity in PCa.

Instead, the biofilm was characterized after 24 h by measuring its aggregate metabolic activity using the tetrazolium salt XTT, as reported previously (Koban et al. 2012).

It is however difficult to quantitatively assess this metabolic activity using [18F]-FDG, as BAT mainly uses lipids as an energy source.

Therefore, a reasonable method to monitor the evolving lymph node (LN) immune response is assessment of LN metabolic activity using standardized uptake value (SUV) as a simple semiquantitative measure of 18F-FDG uptake [3],[4].

Streptococcus mutans biofilms were characterized by measuring their aggregate metabolic activity using the tetrazolium salt XTT, and both 24 h biofilms and biofilm forming culture conditions were amenable to this analysis approach.

We evaluated the effects of cisplatin-functionalized silica nanoparticles and cisplatin alone on cellular metabolic activity using the MTT assay after 24, 36, and 48 h, and the results are presented in Fig. 7a.

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It is well known that metabolic activity uses oxygen; therefore, blood in the vicinity of metabolically active tissues will have a different proportion of oxygenated to deoxygenated haemoglobin (Hb) than that surrounding quiescent tissue.

In the framework of intensity-modulated radiotherapy, Stéphanie Blockhuys (Ghent University Hospital, Ghent, Belgium) showed spatially fractionated irradiation effects on cell metabolic activities, using MTT conversion by cellular dehydrogenases.

To determine the enzyme activity, we used the calorimetric MTT (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide) metabolic activity assay using cell growth determination kit (cat # CGD-1, Sigma-Aldrich) carried out according to the manufacturer's instructions.

Cell growth was assessed by a colorimetric metabolic activity assay using 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) solution.

The assessment of cellular metabolic activity by using PET imaging and F-FDG infusion along with the evaluation of lung perfusion and ventilation, as performed by de Prost and colleagues [ 1], is an informative, non-invasive approach that provides useful data regarding regional differences in cellular metabolic rate.

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