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In this paper, details of methods of metabolic measurement using PET, [18F]fluorodeoxyglucose ([18F]FDG) and [15O]radio-labelled water ([15O]H2O) will be explained.
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Metabolic measurements using indirect calorimetry can be useful in determining nutritional needs and designing nutritional regimens in the pediatric patient in a PVS.
Discussion: The metabolic measurements using indirect calorimetry validated the low caloric needs in a child in PVS.
Assessment/Results: Nutritional assessment based on metabolic measurements using indirect calorimetry showed that the child required significantly fewer calories than predicted by conventional formulas (152kcal/d vs 998kcal/d).
Metabolic flux analysis can be experimentally verified by flux measurement using isotopically labeled carbon.
Systolic blood pressure was measured using a tail cuff blood pressure analyzer (Apollo-2AB Blood Pressure Analyzer, Model 179-2AB, IITC Life Science, Woodland Hills, CA, USA) at week 12. Urine samples were collected over a 24 h period in metabolic cages for catecholamine measurements using 1 M HCl as a preservative.
Oxygen consumption (VO2) will be continuously measured using a metabolic measurement system, which is performed by a breath-by-breath gas analysis (Oxycon Pro, Jaeger).
All metabolic measurements were conducted using proven methodology [ 17] under standardized conditions used in our laboratory.
We validated the DLW method for measuring metabolic rates in five rhinoceros auklets (Cerorhinca monocerata) compared with simultaneous measurements using the respirometric method.
An automated metabolic measuring cart (Truemax 2400 Metabolic Measurement System, Parvomedics, Salt Lake City, UT) was used to measure rates of O2 consumption (VO2) and CO2 production (VCO2).
VO2 was measured using an AEROSPORT KB1-C metabolic measurement system.
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