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The optimum combination of inorganic salts for carotenoids yield was evaluated with the non-linear optimization algorithm as follows: MgSO4 0.12 g/L, Na2HPO4 2.05 g/L, FeSO4 0.03 g/L, Na2CO3 2.22 g/L, and the other components of fermentative medium were kept the same as basal medium (citric acid 8.1 g/L, NH4Cl 3.5 g/L, corn syrup 1.8 g/L).

Dishes with M16 medium were kept at 37°C in a 5% CO2/air mixture, and dishes with M2 medium were kept at 37°C in air.

Sodium phosphate buffer (pH 7.0) for dissolving lipase, reaction temperature of 37°C, and cyclohexane as reaction medium were kept constant throughout the entire experimentation.

SiO2 concentrations in the dissolution medium were kept below 30 ppm to ensure in sink condition (free dissolution of the SiO2 matrix).

The samples in 2 ml cryovials containing virus transport medium were kept at 4°C and transported twice per week to the Pasteur Centre of Cameroon (PCC) for analysis.

Similar(55)

The temperature of the growth medium was kept constant at 25° in all the experiments.

A specified amount of culture medium was kept in the same condition as a negative control.

The medium was kept at 4 °C overnight and then centrifuged at 12,000 × g for 30 min.

The pH of the medium was kept constant during the experiments, showing that molasses presents a buffering effect.

The concentration of DMSO in the medium was kept 0.1% so that it has no effect on the cell proliferation.

The reaction medium was kept under stirring for 2 days in an argon atmosphere at room temperature.

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