Sentence examples for medium were maintained from inspiring English sources

Culture flasks with medium were maintained in a CO2 incubator at 37 °C and 5% CO2 as per the protocol described in Nikolic et al. (2012).

For the repeat range finding test and the definitive test, a reduced pH was not required and the holding and test medium were maintained at a pH range of 6.0 8.5.

Vero cells (EC ACC, 88020401) adapted to grow in serum-free medium were maintained in serum-free OPTIPRO medium (Invitrogen).

Briefly, hanging drops containing 10 cells in 20 µl culture medium were maintained for 2 days under the lid of bacteriological dishes filled with PBS.

Two sets of controls, one set of plates with distilled water used to dissolve ANE, and a second set with equal amount of methanol used to dissolve LPC (100 μl L-1 medium) were maintained.

Two fragments of a previous crop in 2% WA of D. flagrans, A. musiformis, C. rosea, and T. esau, with approximately 4 mm being transferred alone or in association with other fungi to Petri dishes of 100 × 15 mm (06 replicates) containing 20 mL of 2% WhA culture medium, were maintained at room temperature and protected from light.

The pH of the growth medium was maintained at 6.0 by computer-controlled addition of 1 M NH4OH.

The moisture content of the medium was maintained at 64±4% (wet weight basis) for trial one and 69±4% (wet weight basis) for trials two and three using a load cell method.

The medium was maintained at pH 7.0.

The medium was maintained at pH 7.5, and the culture vessel was rotated at 400 rpm.

The pH of the medium was maintained at 10 and stirring for 12 h.