Sentence examples for medium was cooled from inspiring English sources

The medium was cooled to 37 °C, and cells were treated for 12 h.

After autoclaving, the medium was cooled to 55°C with slow stirring.

The culture medium was cooled to 18°C and supplemented with 0.5 mM IPTG.

The medium was cooled to 26°C and then supplemented with IPTG to a final at 1.0 mM concentration.

Medium was cooled to 0°C by adding ice (stored at −20°C) and simultaneously cycloheximide was added to a concentration of 100 µg/ml to quickly halt translation and freeze translating ribosomes in place.

After the basal medium was cooled from the autoclave, the Mn(IV) oxide stock was added anaerobically to a final concentration of 20 mM in basal medium with 10 mM acetate.

After 4 h, the cultures growing in LOC medium were cooled to room temperature, harvested by centrifugation (6,100 × g, 10 min), and washed three times at room temperature with 1X C. bescii partial base salts [ 6] to remove the rich media.

Important factors for choosing a suitable liquid medium are cooling ability and the minimal evaporation during arc discharge [18].

Once the medium was cool, 4.2 g of NaHCO3 and 0.5 g of L-cysteine·HCl·H2O was added per litre.

Dust grains increase the molecular-formation rate by two orders of magnitude compared to the case without dust (e.g., Hollenbach and McKee, 1979), and the interstellar medium (ISM) is cooled efficiently by molecules and dust.

After the sterilized medium had been cooled to 42°C, it was inoculated with 10% (v/v) of the yeast preculture and incubated with agitation at 50-80 strokes per min (spm) in a reciprocal shaker (Bioshaker TA-25R, Takasaki Scientific Instruments, Saitama, Japan) and 42°C.