Sentence examples for medium were cooled from inspiring English sources

After 4 h, the cultures growing in LOC medium were cooled to room temperature, harvested by centrifugation (6,100 × g, 10 min), and washed three times at room temperature with 1X C. bescii partial base salts [ 6] to remove the rich media.

When all the starting material was consumed, reaction medium was cooled down to room temperature and filtered.

In the expression with pCold II vector, the medium was cooled on ice for 30 min before the addition of IPTG.

The medium was cooled to 37 °C, and cells were treated for 12 h.

After autoclaving, the medium was cooled to 55°C with slow stirring.

The culture medium was cooled to 18°C and supplemented with 0.5 mM IPTG.

The medium was cooled to 26°C and then supplemented with IPTG to a final at 1.0 mM concentration.

After the basal medium was cooled from the autoclave, the Mn(IV) oxide stock was added anaerobically to a final concentration of 20 mM in basal medium with 10 mM acetate.

The medium was cooled to 50°C after autoclaving, before approximately 25.0 to 30.0 mL of the medium was poured into 15 × 100 mm plastic petri dishes at a uniform depth of 4 mm.

Important factors for choosing a suitable liquid medium are cooling ability and the minimal evaporation during arc discharge [18].

Once the medium was cool, 4.2 g of NaHCO3 and 0.5 g of L-cysteine·HCl·H2O was added per litre.