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Exact(6)
Only for the plates used for germination, this basic medium was complemented with 5 g L−1 sucrose.
For the generation of B-cell blasts, medium was complemented with 100 U/ml of mIL-2, 10 µg/ml mIL-4, 10 µg/ml mIL-5 (all Peprotech) and 20 µg/ml LPS (Sigma-Aldrich).
To further understand the effects mediated by reelin or NRG1, the culture medium was complemented with pathway inhibitors such as: LY294002 (inhibitor of PI3K; 50 µM, Calbiochem, San Diego, CA), PP2 (a Src kinases inhibitor; 10 µM, Calbiochem), TDZD-8 (a GSK-3β Inhibitor I, 56 µM, Calbiochem), or SP600125 (a JNK Inhibitor II, 10 µM, Calbiochem).
The murine pre-B-cell line 70Z/3 was maintained under the same growth conditions, but the R-10 medium was complemented with 50 μ M mercapto-ethanol.
The growth deficiency of C. glutamicum IB2103 in CGXII minimal medium was complemented by transformation with plasmid pIB4000 carrying the cloned dtxR gene.
In addition, MCF-7 medium was complemented with 10 mM MEM Non-Essential Amino Acids Solution (NEAA, Mediatech, Inc., Manassas, VA), and with 10 ug/ml Bovine Pancreas Insulin from Sigma Aldrich (St . Louis MO).
Similar(54)
Maecker et al. used the optimized freezing medium from Disis et al., which was complemented with HSA (6.25 %) in HLA-peptide multimer staining, cytokine flow cytometry and ELISPOT experiments.
To generate SILAC conditions, normal DMEM medium deficient in arginine (R) and lysine (K) was complemented with stable isotope-encoded arginine and lysine as described previously [ 15].
This work was complemented by theoretical modeling using an effective medium approach [ 115].
Measurement of particles, fields, and dust in the interstellar medium will be complemented by search for complex organic molecules and remote sensing capabilities in various spectral bands.
This control experiment demonstrated that the growth deficiency of the dtxR mutant C. glutamicum IB2103 in CGXII minimal medium can be complemented by plasmid pIB4001 but not by the empty cloning vector pEC-XK99E (data not shown).
Related(20)
medium was completed
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medium was complete
medium was refreshed
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medium was purchased
medium was collected
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medium was harvested
medium was named
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