Sentence examples for medium was combined from inspiring English sources

The wash medium was combined with the supernatant of the previous step.

Fresh medium was combined 1 1 (v/v) with filtered conditioned medium from earlier BEC cultures.

Cells, washes and culture medium were combined.

Cells floating in the medium were combined with the adherent cell layer, which was then trypsinized.

Equal amounts of the total RNA from cultures grown in each medium were combined to create a single pool of total RNA.

Oligomer Oligofectamine™ complexes and aliquots of 2×10 HeLa cells in 2 ml of culture medium were combined and incubated for 5 min at room temperature.

Hippocampal neurons were transfected on DIV3 (for morphometry) or DIV5-8 (for videomicroscopy) as follows: for each coverslip, plasmid DNA (2 μg) and Lipofectamine 2000 (1.25 μl, Life Technologies) in Neurobasal medium were combined and incubated for 30 min. After the addition of complete Neurobasal medium containing B27 supplement, the mix was applied onto the neuronal culture for 3 hr at 37°C.

For each well, Lipofectamine™ 2000 was diluted in 100 μl of serum free -GHT medium and was combined with different amounts of the plasmid (500 ng-5 μg) in 100 μl of serum free -GHT medium, always maintaining a 2 1 ratio (μl of Lipofectamine : μg of plasmid).

Briefly, cells were incubated with 1 ml of the serum-free medium DNA/GenePorter transfection reagent complex, which was prepared by mixing 2  μg DNA with 0.5 ml serum-free medium, and this was combined with 10  μl of GenePorter transfection reagent mixed with 0.5 ml serum-free medium, followed by 30 min incubation at room temperature.

An aliquot from the culture containing cells and medium (500 μl) was combined with 465 μl 4 N NaOH and vigorously mixed for 1 min. The mixture was neutralized by adding potassium phosphate (1 M pH 8.0) and centrifuged for 5 min at 13,000 × g at room temperature.

Data presented here showed that all four silicone components tested were capable of altering the activity of purified transglutaminase; purified trypsin activity was altered only when medium molecular weight PDMS was combined with silicone oil.