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Exact(7)
Conversion to active vitamin D was confirmed by the presence of 1,25D3 in the cell culture supernatant at a concentration of >0.4×10−9 M. The concentration of 1,25D3 in culture medium from untreated cells and 25D3 supplemented medium without cells was below detection limit (<0.02×10−9 M).
In contrast, conditioned medium from untreated cells resulted in well-formed capillary-like structures by the 48 hr time point.
We next examined the endothelial-cell proliferative activity of conditioned medium from untreated and heat-shocked HT-1080 cells.
This protective effect was in addition to the basal protection mediated by conditioned medium from untreated astrocytes.
Conditioned medium from untreated (lane 1, Fig. 7) and control siRNA treated cell (lane 2, Fig. 7) contain the same amount of total MMP-2.
There was no aggrecan-degrading activity present in conditioned medium from untreated epiphyseal chondrocytes, and the inclusion of EGTA eliminated aggrecan-degrading activity that was otherwise present in cultures treated with ionomycin alone.
Similar(53)
Highly enriched neuron cultures exposed to glutamate-containing medium transferred from dbcAMP-treated astrocytes were two to four times less likely to die than cells exposed to glutamate-containing medium transferred from untreated astrocytes.
This rescue effect did not occur when UV-treated cells were incubated with conditioned medium collected from untreated cells (P=0.341, one-way ANOVA test with Dunnett's post hoc test; Figure 1a), indicating a contact-dependent mechanism.
Freshly isolated neutrophils were pretreated with Ly294002 (20 μM) or Bay 11-7085 (1 μM) or vehicle control for 45 minutes at 37°C, washed, and cultured for 24 hours in fibroblast-conditioned medium (FCM) from untreated rheumatoid arthritis synovial fibroblasts (RASF) or from rheumatoid arthritis synovial fibroblasts stimulated with TNFα and IL-17 (RASFIL-17/TNF).
To determine whether this cell death induction of VSMC by monocytes was contact dependent, VSMC were cultured with conditioned medium from monocytes untreated or pretreated with LPS for 2 h.
Cellular medium from treated or untreated MLE-15 and BAL fluid from the mice were analyzed by a PlGF ELISA kit (R&D Systems) according to the manufacturer's instructions.
Related(14)
medium from uninfected
medium from raw
media from untreated
medium from tailbud
medium from p2ΦC31-transfected
medium from live
medium from apoptotic
medium from PI16-overexpressing
medium from gastric
medium from traditional
medium from acrylic
medium from unstimulated
medium from constrained
medium from transfected
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