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Normal embryos were raised in 0.003% phenylthiocarbamide in embryo medium from tailbud stage.
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The gene encoding alpha-2-HS-glycoprotein (also named fetuin) steadily increases in expression from tailbud stage up to metamorphosis.
In case of PGCs isolated from tailbud stage embryos, however, the amount of migratory cells was increased to approximately 50%.
We also used an under-agarose migration assay described above to test migratory abilities of PGCs isolated from neurula stage embryos (developmental stage 17 19) and to compare those to PGCs isolated from tailbud stage embryos (developmental stage 28 30).
We quantified cellular adhesiveness of PGCs by single-cell force spectroscopy (Friedrichs et al., 2010), revealing a stronger attachment to their environment during pre-migratory stages of development in comparison to PGCs isolated from tailbud embryos.
It also prevents the medium from growing in some ways.
This medium from Boston had a bizarre suggestion.
Is that asking too much from the medium, or from the technology of the medium?
Ciona intestinalis Eph, ephrin and lamα3/4/5 cDNAs were obtained by RT-PCR from total RNA isolated from early tailbud stage embryos.
Flow transport in fractured medium differs from that in porous medium, an intergranular porous medium.
Projections were also absent from mid tailbud (Figure 6O,S) and late tailbud (Figure 6P,T) stage embryos, suggesting that formation of these projections is impaired in sna>Bix embryos, rather than being delayed in onset.
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