Sentence examples for medium from transfected from inspiring English sources
The beads were then washed with PBS and incubated with 5 ml of conditioned medium from transfected N2a cell to which 0.1 % Triton X-100 was added for 30 min. Bound beads were washed sequentially with 0.1 % and 0.05 % octyl glucoside (Sigma-Aldrich, St . Louis MO) followed by water.
At 48 h post-transfection, the virus-containing cell culture medium from transfected cells was filtered with 0.45 μm filters and used to transduce the SiHa cells or the control C33A cells at 60% confluence.
The day later, the conditioned differentiation medium from transfected 10T1/2 cells was added to C2C12 cells (differentiation condition day 0).
After 48 h the medium from transfected cells was collected.
SEMA3a secretion was evaluated by Western blot of conditioned medium from transfected FG cells, utilising anti-SEMA3a antibody at a 1 : 200 dilution.
The recombinant ovine IFN-gamma and ovine IL-12 were prepared as a serum-free conditioned medium from transfected CHO cells, according to a protocol described for the production of recombinant ovine IL-4 [ 39].
After chicken osteoclasts were cultured for 5 d in a medium containing supernatant from transfected CEFs, the percentage of osteoclast apoptosis was increased significantly, the concentration of TRAP, the area of lacunae on bone flaps and calcium concentration were decreased significantly in the pcDNA3.1/OPG group compared to the control group and the pcDNA3.1 group.
The medium from cells transfected with RKRmBmp2/HA contained as much HA-tagged BMP2 growth factor as medium from cells transfected with the wild type wtBmp2/HA plasmid, indicating that the RKR to AAA did not disrupt synthesis or secretion of the conventional BMP2 growth factor).
Aliquots of medium from the transfected wells were collected 24 h post transfection to measure luciferase activity, and normalized against the Relina activity in the same sample following the procedure previously described.
Two days later, the conditioned differentiation medium of C2C12 was replaced by new conditioned differentiation medium from freshly transfected 10T1/2 cells (differentiation condition day 2).
We have found that S100A14 was secreted into the extracellular medium from S100A14 transfected EC9706 stable clones and KYSE180 cells, and extracellular S100A14 might regulate its endogenous expression, creating a positive feedback loop.
