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In addition, we used conditioned medium from unstimulated macrophages.
We prepared a conditioned medium of macrophage cell line, RAW264, activated with lipopolysaccharide (LPS) for 24 h; (hereafter, CM-LPS. A conditioned medium from unstimulated RAW264 cells was used as the control (CM-LPS.
The levels of sTNF-RII in culture medium from unstimulated T cells were also about three times greater than those of sTNF-RI, although this increased to approximately five times greater after stimulation of T cells with PHA.
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Conditioned medium from cultures of unstimulated mature osteoclasts contained a variety of chemokines, including MCP-1/CCL2, GROα/CXCL1 and IL-8/CXCL8 (Fig. 1A), indicating that osteoclasts had the capacity to recruit immune cells, including T cells and NK cells (via MCP-1/CCL2), and granulocytes (via GROα/CXCL1 and IL-8/CXCL8).
We investigated whether there was any association between polymorphism in the TNF receptor genes and levels of sTNFRs released into the culture medium of unstimulated and stimulated T cells from RA patients.
Furthermore, the GDF15 concentration in the culture medium of diosgenin-stimulated HEL cells significantly and progressively increased from 120 to 144 h relative to that in the culture medium of unstimulated cells (Fig. 3F).
Vascular smooth muscle cells were then co-cultured with microvesicles isolated from conditioned medium of either unstimulated monocytes or monocytes stimulated with LPS for different periods of time.
In contrast, only 15±6 HIV-1 immunospots were obtained from unstimulated T cells.
Thr855 phosphorylation of MYPT from unstimulated platelets was not decreased in patients with septic shock (n = 22 to 24).
To assess the role of immature oocyte collection from unstimulated ovaries as a potential source of oocyte donation.
Total RNA from unstimulated or inflammatory cytokine-stimulated ARPE cells were isolated and analyzed for OX40L expression by reverse transcription-polymerase chain reaction.
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