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The assays in semi-synthetic medium containing equal amounts of glucose and fructose and inoculated with the strains of S. cerevisiae (PE-2 and the rough strain) separately have revealed that both sugars were consumed concomitantly by the yeasts with different rates.
These cells were cultured in a medium containing equal volumes of Dulbecco modified Eagle medium (DMEM) and F12, with an additional 2 ng/ml recombinant human EGF (R&D Systems, Minneapolis, MN), 1 µg/ml bovine insulin, 0.1 µg/ml cholera toxin, 0.5 µg/ml hydrocortisone, and 10% FBS (all except EGF are from Invitrogen-GIBCO, Carlsbad, CA).
The cellulase activity (CMCase activity) in spent SAFS and SIFS hydrolysates reached 5.2 U/mL (87 nkat/mL), which was similar to the activity level obtained in a reference medium containing equal amounts of reducing sugar.
Media were exchanged for serum free PrEGM (Cambrex) and following 48 h incubation, samples of culture medium containing equal total protein concentrations were analyzed by denaturing Western blot as described above.
The cells were cultured in medium containing equal amounts of Dulbecco modified Eagle medium; Mammary Epithelial Growth Medium (Lonza, Walkersville, MD, USA) supplemented with bovine pituitary extract (70 μg/mL), human epidermal growth factor (5 ng/mL), insulin (5 μg/mL), and hydrocortisone (0.5 μg/mL); and 2% fetal bovine serum.
were used to prepare primary chicken embryo cells which were grown in a medium containing equal quantities of Leibovitz and McCoy's medium with 5% fetal bovine serum, 100 IU of penicillin G/ml, and 100mg of dihydrostreptomycin/ml in 150-mm-diameter tissue culture dishes.
Similar(54)
HAECs at P1 were cultured for 7 days in medium containing an equal volume mix of HAM's F12: Dulbecco's Modified Eagles Medium (1 1) supplemented with different concentrations of EGF (0, 5, 10, 20, 30 and 50 ng/ml EGF) in reduced serum.
In a previous study, we observed the characteristic CCR in E. coli cells that were grown in batch culture in a medium containing an equal mix of five different carbon substrates [ 7].
Conditioned medium and cell lysates containing equal amounts of protein were electrophoresed on 10% SDS PAGE gels under nonreducing conditions and transferred to nitrocellulose membranes.
Briefly, samples containing equal amounts of protein (25 μg) from lysates of the LNCaP sublines cultured in either standard medium or CSM were electrophoresed on an SDS polyacrylamide gel and transferred to a nitrocellulose filter.
After being washed twice with PBS (pH 7.4, containing 137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, and 8.1 mM Na2HPO4), the cells were incubated in fresh culture medium containing TSA (25 ng/mL equal to 82.5 nM) alone or in combination with quercetin, and Q3G (5 μM) for indicated time.
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