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They are successfully differentiated into osteoblasts in the medium containing dexamethasone, ascorbic acid, and β-glycerophosphate.

A nonunion model was made in rat femur, and rat bone marrow cells were cultured in medium containing dexamethasone and ascorbic acid phosphate to create a cell sheet that could be scraped off as a single sheet.

To determine whether OC cells undergo adipogenesis, cells were cultured in medium containing dexamethasone, isobutyl-methylxanthine and indomethacin.

In both cell populations, this plateau was reached around day 14 when cells were cultured in medium containing dexamethasone.

Osteogenic differentiation was induced using either a classical osteogenic medium (containing dexamethasone) or medium containing autologous growth factors (PL) that was shown to promote MSCs differentiation [ 19, 28].

Hormonally defined medium containing dexamethasone, glucagon, insulin, and EGF enhances integrity of the spheroids and maintains production of albumin, glucokinase, and transferrin for up to 60 days in suspension culture (Tong et al., 1990; Yuasa et al., 1993).

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In pro-calcific medium (PCM) containing dexamethasone, AVICs differentiate into osteoblasts capable of calcium deposition and express markers of osteoblast formation runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP).

When the cells reached 80% conflucence, culture medium was replaced with the osteogenic medium containing 10−7 M dexamethasone (Sigma), 10 mM β-glycerophosphate (Sigma), and 50 µg/ml vitamin C (Sigma).

To study the osteogenic differentiation of BMSCs, osteogenic medium containing 10 8 M dexamethasone, 50  μ g/mL L-2-ascorbic acid, and 10 mM β -glycerophosphate were used.

Normal human cells were cultured in SkBM-2 medium containing hEGF, Dexamethasone, L-glutamine, FBS and Gentamicin/Amphotericin-B in pre-mixed aliquots as per manufacturer's instructions.

At the end of the different treatments, mice were killed and BMCs from the femora were flushed out in osteoblast differentiation medium containing 10−7 M dexamethasone (bone marrow differentiation medium).

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