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The recorded value of 18 mg/(g h) is comparable to the maximum value for the P. putida KT42C1 pVLT31_reportedported by Wittgens et al. (2011) and about 38% for the very similar construct P. putida KT2440 pSynPro8_rhlAB grown on a complex rich medium containing additional glucose (Tiso et al. 2016).
Expression of hfq exhibited a 5.21-fold decrease after prolonged growth (4 hr) in medium containing additional 1% NaCl.
The HEK or Flp-In-293 clones expressing recombinant human CCN2/CTGF (rhCTGF) were cultured in the same medium containing additional 150 µg/ml Hygromycin B (Roth, Karlsruhe, Germany).
For sucrose treatment, the seedlings were subcultured in the previously described medium or the previously described medium containing additional 90 mM/L sucrose for 7 days.
Embryo development was induced by transfer of the cells to plant growth regulator-free medium [ 18], either growth regulator free standard medium or growth regulator free "U-medium" [ 65], which is a rich supplemented medium containing additional organic compounds and micro-elements.
Similar(55)
Similar results were obtained from growth on MTC medium containing Avicel (Additional file 4).
Moreover, the C718.5 mutant grew only poorly in high-iron medium when compared with a control medium containing no additional iron sources, and it was more-easily killed by exposure to high-iron conditions and H2O2 than was the parental strain [ 33].
Tamoxifen resistant variant cells (MCF-7-TamR) derived from MCF-7 cells were continuously cultured in the medium as described above containing additional 10-7 M 4-OH Tam (Sigma-Aldrich, St Louis, MO, USA) for at least six months, along with the parental MCF-7-cells under identical culture conditions except that the control cells were treated with 0.1% ethanol.
Extraembryonic endoderm (XEN) cells were derived in medium containing serum without additional growth factors.
Indole-3-acetic acid added at 2 h to the medium containing TEA induced additional membrane hyperpolarization by 25.4 mV (Table 2), suggesting that both processes are synergistic.
For the UCSD-AML1 cell line, the used medium contained an additional 10 ng/ml of GM-CSF (Promocell, UK).
Related(14)
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