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Compared with Group 1, the mean fluorescence density of the actin cytoskeleton in the other three groups was 28.9%, 45.7%, and 33.0%, respectively.
Platelet-bound anaphylatoxins were detected by fluorescence microscopy measuring the mean fluorescence density of the thrombi.
We established that the fluorescence intensity was within the linear range and used mean fluorescence density as a measure of protein concentration.
(B – D ) Percentage of the nuclear area (B ), the mean fluorescence density (C ), and the total green fluorescence (D ) segregated to the mother cell in telophase cells with and without plasmids using different nuclear pore markers (N > 25 cells, ***p < 0.001, **p < 0.01).
To allow comparisons between stereocilia from different samples, fluorescence densities of individual ROIs (from the first and second row) from a single hair cell were normalized to the mean fluorescence density of the first row measured from the same hair cell.
Similar(55)
Alexa 647 and DAPI channels were photographed separately and relative mean red fluorescence densities were determined in 30 areas of cytoplasm and cell nucleus in all the experimental settings using the Image J software (NIH, Bethesda, MD, USA).
Similarly, the mean fluorescence intensity (integrated density divided by area) was plotted as well as the radius of the G1 nuclei, measured drawing a line through the nucleus at its broadest area using ImageJ, dividing it by two and plotting it against the age of the cell.
Therefore, α-flag FITC (2.5 µg/mL) was added to cells to quantify the percentage of cells with surface-expressing GPCRs and the density (mean fluorescence intensity; MFI) of GPCRs on the surface of those cells.
Similar differences were observed in comparison of the density (mean fluorescence intensity, MFI) of these molecules expressed on the surface of these cells.
The average pixel density (mean fluorescence intensity, MFI) within the selected area was calculated.
The frequency and density (mean fluorescence intensity, MFI) of CD38 or HLA-DR expressing subsets was determined by a histogram plot whereas the percentage of the CD38+ HLA DR+ cells were determined by two-color dot plot (FITC versus PE).
Related(20)
average fluorescence density
mean fluorescence intensity
mean fluorescence rate
mean fluorescence intensities
mean water density
mean tag density
mean wood density
mean population density
mean map density
mean contig density
mean larva density
mean power density
mean parasite density
mean cell density
mean fluorescence channel
mean volume density
mean staining density
mean resource density
mean count density
mean mosquito density
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