Membrane protein deficiency was determined when patients had ≥10% and ≥2 standard deviation difference relative to the mean stain density for each fraction in healthy control subjects (n = 3); controls were run on the same gel as the study subjects.
Evaluation of proET-1, PLT granule staining density, TAT and fibrinogen (mean value with standard deviation) established in the group with end-stage CKD.
The decrease of HLTF staining was statistically significant during thyroid tumor progression in terms of both the percentage of mean optical density (MOD), which corresponds to the mean staining intensity (Kruskall-Wallis: p < 0.0005), and the labelling index (LI), which corresponds to the percentage of immunopositive cells (Kruskall-Wallis: p < 10−6).
The relative staining densities in the basal and luminal thirds of the epithelium of each subject were determined by taking the mean pixel grey-scale density value from 5 separate equal area fields in the relevant areas.
The staining intensity was graded according to the mean optical density: 0, no staining; 1, weak staining (light yellow); 2, moderate staining (yellow brown); and 3, strong staining (brown).
The proportion of DUSP-9 expressing tumor cells was scored as follows: 0, no positive cells; 1, <5%; 2, 6%-25%; 3, 26%-50%; 4, 51%-75%; and 5, >75% according to Tsuchiya et al. The staining intensity was graded according to the mean optical density [ 10- 12]: 0, no staining; 1, weak staining (light yellow); 2, moderate staining (yellow brown); and 3, strong staining (brown).
The integrated optical density (IOD) as the positive-staining density was measured as reported previously [ 24].
E-cadherin-positive surfaces, but not the staining intensity (mean optical density), were significantly different between IBC and non-IBC (35.4 and 10.3%, respectively; P<0.001, Mann–Whitney) (Table 1).
Nuclear ER concentration by image analysis (mean optical density of stain) was not associated with the DCC measurement of ER concentration, P greater than 0.05.
