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Background-corrected total lung counts were calculated from the lung mean count density above the exclusion zones.
The mean count density per pixel in each region was calculated and corrected for the effects of decay.
The background-corrected counts for the entire lung were calculated as the product of the background-corrected lung mean count density and the total CT lung volume on SPECT/CT.
This was further divided by the CT lung volume above the exclusion zone to obtain the background-corrected lung mean count density (counts/cm3), which we assumed to be representative of the entire lung.
The H/M ratio was calculated by dividing the mean count density (i.e. counts/pixel) within the cardiac ROI by the mean count density in the mediastinal ROI.
In this formula H represents the mean count density in the cardiac ROI and M represents the mean count density in the mediastinal ROI in the early (e) and (l) late images.
Similar(54)
The estimated value in each ROI was expressed as a percentage of the mean cerebellar count density.
Acquisition at rest was stopped when the mean left ventricular count density was approximately 130/pixel (matrix size: 64×64; regular field of view camera, zoom 1.3).
Microvessel density was defined as the mean count of microvessels per 0.26 mm field area.
To allow comparison of regions with different cell densities raw counts from each area were normalized by dividing them by the mean count for that area across groups and multiplying by 100.
The numerical densities of elongated spermatids were averaged across the 3 testicular cross sections assessed to obtain a mean count for each individual animal (37).
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