The mean contig size was 607 bp.
These were assembled into 1,831 contigs (mean contig depth = 4.1 sequences).
The mean contig length and N90 contig length are also both shorter by the 454 data.
The mean contig length increased to 454 bases.
Of these, median coverage was 26 reads/contig (mean coverage = 173.73 reads/contig; SD = 677.99), and median contig length was 406 bases/contig (mean contig length = 439.7; SD = 429.9).
There was no relationship between chromosome length and contig density (F1,28 = 1.35, p = 0.26; R2 = 0.04).
We compared the four assemblies using the following standard metrics: total number of reads used in the assembly, number of contigs generated, N50 length of contigs, number of contigs, mean contig length, and summed contig length.
The first group of index included total number of reads used in the assembly, number of contigs generated, N50 length of contigs, number of contigs, mean contig length, and summed contig length (Table 2).
In contrast, using annotated contigs, the contig number decreased greatly, and the mean contig length and N50 value each increased (Table 2).
Maximum, minimum, and mean contig length, N50 and total bases were calculated from the contigs after clustering and removal of those contigs < 200 bp.
