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Exact(7)
Overall, this would result in a maximal cell number of 3.6×108 to 7.1×1011 for MSCM1 and 1.1×108 to 1.7×108 for MSCM2.
The cell numbers were converted into percentage by dividing them with the maximal number of cells used in this experiment, as was the luciferase activity from each group by dividing the reading with the reading from the maximal cell number group.
The estimated effective dose (ED50) stimulating 50% of the maximal cell number response was determined from MTT proliferation curves.
The constitutive over-expression of c-myc, has clearly resulted in an overall increase in proliferation rate but also an increased maximal cell number under batch conditions.
Cell engineering strategies to improve maximal cell number would be a major step towards reaching the goal of increased product titre.
The increase in IVC would be an advantage in bioprocess systems where the increase in overall maximal cell number usually associated with more product formation if cell production rate is not significantly decreased.
Similar(52)
Volumetric EPA and DHA productivity was calculated from cellular PUFA content, specific growth rates and maximal cell numbers (Table 4).
Hit conditions were defined as those ECMP combinations that supported maximal cell numbers, as well as GFP expression.
Over the past decades, the increase in maximal cell numbers for the production of mammalian derived biologics has been in a large part due to the development of optimal feeding strategies.
We have established an optimised RNA isolation workflow specifically designed to yield maximal RNA from minimal cell numbers.
Similar to the increase of the IFN-γ level in AAF/PH-treated rats, Stat-1 was already activated at days 1 and 3 after PH, before maximal oval cell numbers occurred.
Related(20)
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