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Maximal cell migration (chemotaxis) was obtained using cell migration media containing ten (10) percent fetal calf serum (FCS).
This is similar to the biphasic model (DiMilla et al., 1991), where an intermediate strength of cell substratum interaction is essential for maximal cell migration.
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Moreover, reduced IFNγ implied that local T cell stimulation by viral antigen was specifically reduced in the absence of TIMP-1 at the time of maximal T cell migration to the CNS.
The combination of estrogen and EGF resulted in maximal stimulation of cell migration and ERK phosphorylation.
In the three HMCL, the maximal increase in cell migration to MCP-1 ranged between 38 and 75%, corresponding with 21 28% of the total number of cells in the upper compartment that actively migrated through the membrane into the lower compartment of the Transwell migration system.
In practice, we found that TNF- α at a concentration of around 100 250 U ml−1 added for 20 24 h produced maximal effects on both cell migration and cell invasion.
Taken together, these results demonstrate that type I collagen promotes a phenotype that is consistent with malignancy in the majority of our pancreatic cancer cell lines, as defined by maximal cell adhesion, proliferation, and migration, relative to the other ECM proteins tested.
Our data showed that leptin (10 500 ng ml−1) treatment significantly enhanced cell migration, with a maximal response at the dose of 500 ng ml−1 (P<0.001 for both cell lines) compared with controls.
A combination of negative pressure and increased doses of lithium synergistically increased Wnt signaling and demonstrated further enhanced cell migration into simulated wound sites, with maximal filling of the simulated wound observed at lithium concentrations of at least 10 mM.
Based on the quantification of the inhibition of cell migration we obtained substantial but not maximal inhibition at 35 μM D4476 (marked in red).
These have allowed researchers to establish a model for cell migration that shows that an intermediate strength of adhesion is required for maximal cell speed (DiMilla et al., 1991; Palecek et al., 1997).
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