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In particular, for cell-based therapies, such methodologies can be used as a means to determine the optimal cell number to be implanted.
250 000 cells/well was determined to be the optimal cell number to be used per well with a further increase in cell number per well diminishing antigen-specific responses (Figure 2).
The optimal cell number was found to be 1 × 10 cells/well.
Optimal cell number per well (5000) was determined in initial titration experiments.
It also allows the clustering run to be performed at an optimal cell number (e.g., 1 5 million cells).
For functionality test, samples desiccated for 15 minutes were chosen, in view of optimal cell number obtained from viability assay.
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For assessment of optimal cell numbers, nine animals were used per condition.
Optimal NK cell number and purity were obtained when culturing with K-562 cells that expressed both IL-15 and 4-1BBL in IL-2 supplemented RPMIL-240 media.
In particular, peripheral NK cells were partially supported by IL-15Rα/IL-15 IL-15Rα/IL-15 IL-15Rα/IL-15poiexpressionnhematopoietic cells, byt both compartmeitherere required for optimal NK cell number [ 121, 126, 128].
The authors propose, therefore, that adenosine signalling restores an optimal β-cell number, rather than increases β-cell proliferation per se.
Having established optimal cytokine kinetics and cell number sensitivity in PBMC, we next evaluated the ability of the qPCR platform to measure ex vivo cervical HIV-specific immune responses.
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