Sentence examples for markers were excised from inspiring English sources

Individual AFLP markers were excised and eluted from the polyacrylamide gel into distilled water to obtain DNA fragments of each marker.

These 85 markers were excised from AFLP gels to develop Carica papaya sex-liked markers (CPSM).

In addition, those dominant bands that were observed in the profiles but that did not match with any of the bands present in the DGGE markers were excised from the gels and used as templates for sequencing analysis.

In addition, notorious DGGE bands that could not be identified by comparison with the DGGE markers were excised from the gels with sterile surgical blades and the DNA was extracted using the protocol of Sanguinetti et al. [ 14].

The bands corresponding to the 8000 M/Z markers were excised and then destained with two washes of 50  μl deionised water, followed by 50  μl ACN/50 mM l−1 NH4HCO3 (1 : 1, v/v), and dried in a SpeedVac concentrator (FisherScientific, Pittsburgh, PA, USA).

The red, cyan, and yellow fluorescent protein markers were excised by digesting 3xP3DsRed1, 3xP3ECFP, and 3xP3EYFY with BglII and BsaI and each was individually subcloned into the BamHI and BsaI sites of pDIE1-TOPO.3 to produce pDIE1DsRed1-TOPO.3, pDIE1ECFP-TOPO.3, and pDIE1EYFP-TOPO.3, respectively.

After electrophoresis, the gel segments containing the chromosomal markers was excised and stained with ethidium bromide to visualize DNA under UV light.

This included size selection using a 6% PAGE Gel; the region between the custom Illumina markers was excised, corresponding to insert sizes of approximately 20 35 nucleotides.

From those, the WSC1 alleles including the selection marker were excised with SalI/BssSI and used to substitute the wsc1::SpHIS5 deletion in strain HSK1.1 by in vivo recombination.

(E) Southern blot analysis of the knock-in hESCs showing that the selection marker was excised from the targeted allele by LoxP/Cre-mediated deletion.

Then, using Flp recombinase the selectable marker is excised, leaving behind the tag of interest and a 34 bp "scar" corresponding to one of the FRT sites.