All InDel markers were designed by Primer 5.0 software.
The two markers were designed based on the insertion/deletion between Nipponbare and Kasalath.
InDel markers were designed based on the references genome of Nipponbare and 9311 reference sequences.
The sequences of the markers were designed using the PREMIRE5.0 software.
Nuclear INDEL markers were designed to distinguish O. meridionalis from Nipponbare (Additional file 2: Figure S2).
Accordingly, microsatellite markers were designed, and their ability to detect polymorphisms was determined.
Furthermore, Sequence Characterized Amplified Region (SCAR) markers were designed envisioning the detection of milk adulteration in processed dairy foods.
Skill level markers were designed to measure the knapper's ability to achieve a series of set goals.
Both CAPS and dCAPS markers were designed to detect G/A SNP using the restriction endonuclease Nla III and Tsp45 I, respectively.
The microsatellite markers were designed for the giant liver fluke, Fascioloides magna, veterinary important liver parasite of free-living and domestic ruminants.
A total of 33,263 microsatellite sites were identified from the C. pseudocerasus genome, among which 30,148 SSR markers were designed.
