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Each Nissl-stained neuron in the ventral horn with a distinct nucleolus and a darkly stained cytoplasm was manually traced using Metamorph Imaging software.

Therefore the third Rrp4 subunit was manually traced using COOT [49] and built from three- to ten-amino acid-long peptide segments of Rrp4.

Filaments were manually traced using ImageJ and deviation from the longitudinal cell axis calculated in MATLAB.

To determine the morphologies of dendritic spines, the outline of the dendrite, including all spines, was manually traced using the signal from a morphology marker (usually GFP).

The outline of the intima was manually traced using Adobe Photoshop, by a blinded observer, to calculate the total area of the vessel.

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An ABMG board-certified clinical molecular geneticist manually analyzed the resulting sequence traces using Sequence Scanner (ABI).

Particles in embryos imaged using DIC optics were manually traced and marked using MetaMorph Imaging System (Universal Imaging Corp .. Movement of nuclei was traced and quantified in histone-eGFP stocks imaged in real-time by using a sobel filter in MetaMorph Imaging System to outline each nucleus in white and contrast nuclei from the black, lower-intensity background (Figure S11).

Instead, filopodia are detected by eye, and length or other spatial information are extracted by manually tracing filopodia using image manipulation software.

For each LV slice, the slice volume of the particular phase was computed by the product of slice thickness and area of the manually traced blood disc using the pixel to area conversion factor 1pixel/0.22 mm.

The manually traced boundary is plotted using a black curve after all other boundaries are drawn.

Tumour volume was measured using manually traced ROIs drawn on high-resolution three-dimensional, post-contrast, fat-suppressed FSPGR images.

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