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Analytes were manually identified using the TargetFinder plug-in of the TagFinder-Software and a reference library of ambient and 13C labelled mass spectra and retention indices (RI) from the Golm Metabolome Database (GMD, http://gmd.mpimp-golm.mpg.de/) [34], [35].
The breakpoints were then manually identified using IGV by matching misaligned reads.
Linkage groups (i.e. chromosomes) within homology groups were manually identified using the linkage phase information.
The boundaries of the inverted repeat (IR) were manually identified using small cut and paste alignments in Geneious.
New putative ncRNAs (i.e., ncRNAs not previously reported or previously identified by Rfam) were manually identified using the genome browser Artemis [ 69].
Intact structures and other TE signatures such as target site duplication (TSD), terminal inverted repeats (TIR), polypurine tracts (PPT), primer binding sites (PBS) and long terminal repeats (LTR) were manually identified using Dotter software [ 50] and ACT.
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Transcription factors were identified using manually curated PFAM domain profiles from DBD: Transcription factor prediction database.
Home blood pressure readings documented in the structured flowsheets in the EMR were identified using manually entered notes in the Comment field (e.g. "at home", "home cuff", "average home readings", etc).. Home blood pressure readings documented in the narrative notes were abstracted using the algorithm described above.
The resulting genome annotation was curated manually, and metabolic pathways were identified using the KEGG pathways [ 82] tool in GenDB.
These connections were identified using a manually curated list of KEGG pathways that excludes overly generic and largely populated pathways (e.g., Pathways in Cancer) that would report spurious relationships (Goel et al., 2014).
For each patient, whole-blood TAC were derived from a VOI that was defined manually within the ascending aorta, as identified using an early PET frame best displaying the blood pool.
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