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Each cell body labeled by both Red Stinger and Alexa Fluor 488 in the central brain was manually marked with a landmark sphere.
The location of the SDS PAGE markers (Rainbow full range, GE Healthcare) on the film relative to the developed bands was manually marked with a pen.
The procedure for specular microscopy was as follow: three images from central cornea were taken of at least 50 contiguous cells and were manually marked with a mouse by the examiner for analysis by a built-in software program.
The first 21 eyes were manually marked with waterproof ink at the slitlamp, whereas the last 29 eyes were marked with waterproof ink using a handheld pendulum marking instrument (G-33776 by Geuder AG, Heidelberg, Germany).
Questionnaires were manually marked with a unique letter to determine which oncology service had distributed the questionnaires; this will allow us to compare participation rates as well as variations in characteristics and responses across metropolitan and rural regions in patients with cancer.
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However, the proposed method achieves better performances for the last two metrics, meaning that it is able to approximate a single manually marked brushstroke with a smaller number of automatically extracted brushstrokes that are larger than those of [9].
The numbers of cells was blindly counted within 0.25 mm of subventricular zone (SVZ), ischemic border zone (IB), and ischemic zone (IZ) using NIH image software, Image J. The immunoreactive cells were manually marked and calculated with Image J.
When the corpus contains documents with manually marked up annotations done by domain experts, they are known as Gold Standard Corpora GSCC).
To present a straightforward comparison with state-of-the-art, we conduct extensive experiments using two publicly available face databases, BIOID database [50] and BUHMAP database [11], with manually marked ground truth positions.
Manually marked and automatically extracted brushstrokes have been provided by and used with the permission of the authors of [9].
In each of these images 4 areas in the region of interest (intima or adventitia) with the highest density of CD3 positive cells were manually marked, using image analysis software (Image Pro Plus, vs. 5.01, Media Cybernetics, Silver Spring, MD).
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