Sentence examples for manually homogenized with from inspiring English sources

Frozen tumors were manually homogenized with homogenization hammer and tissue powders were resuspended in 500 μl of PBS.

Liver biopsy samples were washed in RPMI-1640 to remove contaminating blood lymphocytes, manually homogenized with a plastic plunger, and treated with DNase (0.002%, Sigma) and collagenase IV (0.02%, Sigma) for 30 minutes, stirring at 37°C.

Heart (washed in E3 water to remove blood cells) and blood (washed in 1X PBS (Bio-Rad, Hercules, CA) and spun down at 2,000 RPM) from the wild-type zebrafish were froze in liquid nitrogen and manually homogenized with a pistil and 18- and 27-gauge needles (Tyco Healthcare/Kendall Mansfield, MA).

Similar to the RT-PCR protocol, the heart (washed in E3 water to remove blood cells) and blood (washed in 1X PBS (Bio-Rad) and spun down at 2,000 RPM) from the wild-type sibling were froze in liquid nitrogen and manually homogenized with a pistil and 18- and 27-gauge needles (Tyco Healthcare/Kendall).

The anterior midguts were then transferred to 100 µL of ice-cold MET solution (300 mM mannitol, 5 mM EGTA, 17 mM TRIS-base/HCl, pH 7.5) in a microcentrifuge tube and manually homogenized with an abrasive glass microhomogenizer during 5 min. 100 µL of ice-cold 24 mM MgCl2 were added to this preparation and the tube content was mixed.

The tissue was then manually homogenized with a potter glass.

Tuberculous-like animal lesions were dissected and manually homogenized, then decontaminated with 4% NaOH for 15 min and centrifuged at 3,000 rpm for 15 min.

Tissue (1 × 2 mm) or cell line lysates were manually homogenized (PYREX homogenizer; VWR, Radnor, PA, USA) and prepared as previously described [ 32].

In brief, the tissue was manually homogenized by four strokes with a Teflon pestle in solution I [230 mM mannitol, 70 mM sucrose, 1 mM EGTA and 5 mM HEPES (pH 7.4)] on ice.

Fifty adult flies were manually homogenized in a microfuge tube with a small pestle in subcellular fractionation buffer (220 mM mannitol, 68 mM sucrose, 20 mM HEPES (pH 7.4), 80 mM KCl, 0.5 mM EGTA, 2 mM Mg(CH3COO 2, and Sigma protease inhibitor cocktail (Sigma #P8340)).

To avoid human factors to the bacterial community composition, the subset of sediment for DNA extraction was manually homogenized but not sieved.